Application Support Knowledge Base

The application support knowledge base (ASK) provides a comprehensive set of technology-specific and application-specific scientific articles, written by our R&D, technical support, and applications teams for scientists, enabling you to access scientific support 24 hours a day/7 days a week, with content-rich information covering assay design and assay development, product selection, detailed sample protocols, sample data, data analysis, tips, troubleshooting tables, video tutorials, and selected citations for common assays developed and run using Revvity technologies.

AlphaLISA

AlphaLISA™ is a bead-based assay technology used to study biomolecular interactions in a microplate format. The acronym "Alpha" stands for amplified luminescent proximity homogeneous assay.

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Alpha EMSA Conversion

Alpha Immunogenicity

Alpha Protein-protein and Protein-nucleic Acid Interactions

Alpha Troubleshooting Tables

AlphaLISA Epigenetic Toolbox Reagents

Alpha Instrument Options and Settings

Alpha Bead Conjugation

AlphaLISA 5 μL vs. 20 μL Sample Volume

Alpha Products and Catalog Numbers

Alpha Antibody Detection and Characterization

Alpha Citations

Bead Selection and Bead Interference

The Hook Effect

Other Alpha Applications

AlphaLISA Immunoassay Kits

Preparation of Analyte-depleted Serum for Alpha Assays

Working with cell extracts and supernatants in Alpha assays

Data Analysis for AlphaLISA Immunoassays

Working with Serum and Other Biological Matrices in Alpha Assays

Create your own Alpha Assay

Determining Kd With an Alpha Assay

AlphaLISA and AlphaScreen No-wash Assays

Buffer Selection for Alpha Assays

AlphaLISA SureFire Ultra

AlphaLISA SureFire Ultra assay kits allow the rapid, sensitive, and quantitative detection of phospho- and total proteins from cells. The AlphaLISA SureFire Ultra assay kits are optimized for enhanced signal-to-noise windows.

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Alpha SureFire No-wash Cellular Kinase Assays

FAQs and Guidelines for Successful AlphaLISA SureFire Assay Setup

DELFIA

DELFIA™ (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. Assays are designed to detect the presence of a compound or biomolecule using lanthanide chelate labeled reagents, separating unbound reagent using wash steps.

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Microplates For Absorbance and Colorimetric Assays

DELFIA products and catalog numbers

DELFIA Time-Resolved Fluorescence Assays

DELFIA Cell Proliferation Assay

Multiplexing DELFIA™ assays

DELFIA Kinase Assays

DELFIA Receptor-Ligand Binding Assays

Label your own DELFIA reagents

DELFIA Citations

DELFIA Immunoassays

DELFIA Instrument Options and Settings

DELFIA Biodistribution

DELFIA Buffers

DELFIA Cell Cytotoxicity Assays

In Vivo Preclinical Imaging

Pre-clinical imaging techniques including  in vivo, ex vivo, and in vitro imaging enable the understanding of disease from cellular to whole animal models and are used in the translation to, and development of therapies in clinical-relevant models.

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In Vivo Imaging

IVISbrite Bacterial Cell Lines

IVISbrite Lentiviral Particles

IVISbrite Bioluminescent Substrates

IVISense Fluorescent Imaging Probes - Targeted

IVISbrite Tumor Cell Lines

IVISense Fluorescent Imaging Probes - Vascular

IVISense Fluorescent Imaging Probes - Activatable

IVISense Fluorescent Dyes

Applications

Best Practices

LANCE

ULight™ dye absorption (excitation) and emission spectra.

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LANCE ULight dye spectra

LANCE Citations

LANCE Indirect 4-Component Kinase Assays

LANCE Classic 3- Kinase Assay

LANCE Epigenetic Assays

Suppliers for other cAMP reagents

Suppliers for other kinase assay reagents

Label Your Own LANCE Reagent

LANCE Products and Catalog Numbers

APC dye spectra

LANCE Eu-W8044-DTA spectra

LANCE Eu-W1024-ITC spectra

LANCE Instrument Options and Settings

LANCE kinase assays optimization

LANCE Ultra cAMP Assays

LANCE Ultra Kinase Assays

LANCE Protein-Protein Interaction Assays

LANCE and LANCE Ultra TR-FRET Assays

LANCE Phosphatase Assays

Microplates

Revvity offers a range of 96-well, 384-well and 1536-well tissue culture treated imaging plates. These include the following:

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Microplates for High Content Analysis and High Content Screening Assays

Microplates and Consumables for Next Generation Sequencing

Microplates For Fluorescence Assays

Microplates for Radiometric Assays

Plate Treatments, Coatings and Applications

Microplates Knowledge Base

Microplate Dimensions, Working Volumes and Packaging

Microplates for Storage

Table of Microplates by Application

Microplates For Luminescence Assays

Microplates For High Content Analysis and High Content Screening Assays

Radiometric

NEN radiochemicals are available in a variety of concentrations, specific activities, buffers, shipping, packaging and storage conditions.

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Thymidine Uptake Assays

DNA and RNA Labeling

Scintillation Proximity Assays

In Vitro Kinase Assays

CAT Reporter Gene Assays

Cell and Metabolic Labeling

Chromium-51 Release Assay

Sulfur-35 Methionine Labeling

Iodine-125 Labeling of Proteins

Sulfur-35 GTP Binding Assays

Radiochemical Calculations

Glucose Uptake Assays

Radiometric Assays and Detection

Radiometric Ligand-Binding Assays

Phosphorylation Assays

Acetylation Assays Using Acetyl CoA

Radioimmunoassays

Phosphorus-32 Cell Labeling Assays

Liquid Scintillation Counting

Which Formulation Should I Choose?

Cell Counting and Image Cytometry

Nexcelom’s leading automated cell counting and image cytometry solutions are now part of Revvity’s portfolio.The portfolio includes the Celigo™ image cytometer, the Cellaca™ MX high-throughput cell counter, and Cellometer™ automated cell counters. Here you can find information to support your use of these instruments together with our comprehensive range of complementary consumables and reagents.

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