Application Support Knowledge Base

The application support knowledge base (ASK) provides a comprehensive set of technology-specific and application-specific scientific articles, written by our R&D, technical support, and applications teams for scientists, enabling you to access scientific support 24 hours a day/7 days a week, with content-rich information covering assay design and assay development, product selection, detailed sample protocols, sample data, data analysis, tips, troubleshooting tables, video tutorials, and selected citations for common assays developed and run using Revvity technologies.

AlphaLISA

AlphaLISA™ is a bead-based assay technology used to study biomolecular interactions in a microplate format. The acronym "Alpha" stands for amplified luminescent proximity homogeneous assay.

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Alpha Antibody Detection and Characterization

Alpha Bead Conjugation

Alpha Citations

Alpha EMSA Conversion

Alpha Immunogenicity

Alpha Instrument Options and Settings

Alpha Products and Catalog Numbers

Alpha Protein-protein and Protein-nucleic Acid Interactions

Alpha Troubleshooting Tables

AlphaLISA 5 μL vs. 20 μL Sample Volume

AlphaLISA Epigenetic Toolbox Reagents

AlphaLISA Immunoassay Kits

AlphaLISA and AlphaScreen No-wash Assays

Bead Selection and Bead Interference

Buffer Selection for Alpha Assays

Create your own Alpha Assay

Data Analysis for AlphaLISA Immunoassays

Determining Kd With an Alpha Assay

Other Alpha Applications

Preparation of Analyte-depleted Serum for Alpha Assays

The Hook Effect

Working with Serum and Other Biological Matrices in Alpha Assays

Working with cell extracts and supernatants in Alpha assays

AlphaLISA SureFire Ultra

AlphaLISA SureFire Ultra assay kits allow the rapid, sensitive, and quantitative detection of phospho- and total proteins from cells. The AlphaLISA SureFire Ultra assay kits are optimized for enhanced signal-to-noise windows.

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Alpha SureFire No-wash Cellular Kinase Assays

FAQs and Guidelines for Successful AlphaLISA SureFire Assay Setup

DELFIA

DELFIA™ (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. Assays are designed to detect the presence of a compound or biomolecule using lanthanide chelate labeled reagents, separating unbound reagent using wash steps.

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DELFIA Biodistribution

DELFIA Buffers

DELFIA Cell Cytotoxicity Assays

DELFIA Cell Proliferation Assay

DELFIA Citations

DELFIA Immunoassays

DELFIA Instrument Options and Settings

DELFIA Kinase Assays

DELFIA Receptor-Ligand Binding Assays

DELFIA Time-Resolved Fluorescence Assays

DELFIA products and catalog numbers

Label your own DELFIA reagents

Microplates For Absorbance and Colorimetric Assays

Multiplexing DELFIA™ assays

In Vivo Preclinical Imaging

Pre-clinical imaging techniques including  in vivo, ex vivo, and in vitro imaging enable the understanding of disease from cellular to whole animal models and are used in the translation to, and development of therapies in clinical-relevant models.

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Applications

Best Practices

IVISbrite Bacterial Cell Lines

IVISbrite Bioluminescent Substrates

IVISbrite Lentiviral Particles

IVISbrite Tumor Cell Lines

IVISense Fluorescent Dyes

IVISense Fluorescent Imaging Probes - Activatable

IVISense Fluorescent Imaging Probes - Targeted

IVISense Fluorescent Imaging Probes - Vascular

In Vivo Imaging

LANCE

ULight™ dye absorption (excitation) and emission spectra.

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APC dye spectra

LANCE Citations

LANCE Classic 3- Kinase Assay

LANCE Epigenetic Assays

LANCE Eu-W1024-ITC spectra

LANCE Eu-W8044-DTA spectra

LANCE Indirect 4-Component Kinase Assays

LANCE Instrument Options and Settings

LANCE Phosphatase Assays

LANCE Products and Catalog Numbers

LANCE Protein-Protein Interaction Assays

LANCE ULight dye spectra

LANCE Ultra Kinase Assays

LANCE Ultra cAMP Assays

LANCE and LANCE Ultra TR-FRET Assays

LANCE kinase assays optimization

Label Your Own LANCE Reagent

Suppliers for other cAMP reagents

Suppliers for other kinase assay reagents

Microplates

Revvity offers a range of 96-well, 384-well and 1536-well tissue culture treated imaging plates. These include the following:

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Microplate Dimensions, Working Volumes and Packaging

Microplates For Fluorescence Assays

Microplates For High Content Analysis and High Content Screening Assays

Microplates For Luminescence Assays

Microplates Knowledge Base

Microplates and Consumables for Next Generation Sequencing

Microplates for High Content Analysis and High Content Screening Assays

Microplates for Radiometric Assays

Microplates for Storage

Plate Treatments, Coatings and Applications

Table of Microplates by Application

Radiometric

NEN radiochemicals are available in a variety of concentrations, specific activities, buffers, shipping, packaging and storage conditions.

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Acetylation Assays Using Acetyl CoA

CAT Reporter Gene Assays

Cell and Metabolic Labeling

Chromium-51 Release Assay

DNA and RNA Labeling

Glucose Uptake Assays

In Vitro Kinase Assays

Iodine-125 Labeling of Proteins

Liquid Scintillation Counting

Phosphorus-32 Cell Labeling Assays

Phosphorylation Assays

Radiochemical Calculations

Radioimmunoassays

Radiometric Assays and Detection

Radiometric Ligand-Binding Assays

Scintillation Proximity Assays

Sulfur-35 GTP Binding Assays

Sulfur-35 Methionine Labeling

Thymidine Uptake Assays

Which Formulation Should I Choose?

Cell Counting and Image Cytometry

Nexcelom’s leading automated cell counting and image cytometry solutions are now part of Revvity’s portfolio.The portfolio includes the Celigo™ image cytometer, the Cellaca™ MX high-throughput cell counter, and Cellometer™ automated cell counters. Here you can find information to support your use of these instruments together with our comprehensive range of complementary consumables and reagents.

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